Journal:

Article Title: Heterogeneous Gene Expression and Functional Activity of Ryanodine Receptors in Resistance and Conduit Pulmonary as well as Mesenteric Artery Smooth Muscle Cells

doi: 10.1159/000127438

Figure Lengend Snippet: RyR subtypes are heterogeneously expressed in freshly isolated mouse RPA-SMCs, CPASMCs and MASMCs. a Expression of the smooth muscle-specific actin was found in nearly all isolated cells from resistance pulmonary arteries. Fluorescence image for actin staining (left) and transmitted light image (right) were simultaneously taken using a Zeiss LSM510 laser scanning confocal microscope. Cells were incubated with a primary antibody for smooth muscle-specific actin and then stained with Alexa Flour 488-conjugated anti-mouse antibody. b Gel electrophoresis reveals expression of RyR1, RyR2 and RyR3 mRNAs in RPASMCs, CPASMCs and MASMCs. The predicted PCR product sizes for RyR1, RyR2, RyR3 and GAP-DH were 81, 77, 83 and 122 bp, respectively. c Graphs show the relative mRNA expression levels of RyR1, RyR2 and RyR3 in RPASMCs, CPASMCs and MASMCs. The relative mRNA expression levels were obtained by normalizing the absolute expression levels of RyR1, RyR2 and RyR3 to that of GAPDH. Data were obtained from 6 separate experiments. * p < 0.05 compared with RyR1 in the same type of vascular SMCs; + p < 0.05 compared with RyR2 in the same type of cells; °p < 0.05 compared with RyR2 or RyR3 in RPASMCs.

Article Snippet: Measurements of [Ca 2+ ] i were made using a wide-field fluorescence microscope (IonOptix Corp., Milton, Mass., USA) [ 12 ].

Techniques: Isolation, Expressing, Fluorescence, Staining, Microscopy, Incubation, Nucleic Acid Electrophoresis

Journal: bioRxiv

Article Title: Phase separation and molecular ordering of the prion-like domain of the thermosensory protein EARLY FLOWERING 3

doi: 10.1101/2023.03.12.532276

Figure Lengend Snippet: Coupled AFM and confocal fluorescence microscopy of ELF3 PrLD -GFP constructs. AFM topography mapping is shown at left, fluorescence of the same sample is shown at middle and stiffness measurements are shown at right. Alignment between the AFM and confocal micro-scope was within 1-2 microns for the two microscopes. Scale bar = 5 μm.

Article Snippet: AFM coupled wide-field fluorescence microscope was developed in-house, allowing AFM imaging and epifluorescence or Total Internal Reflection Fluorescence (TIRF) imaging (Supplementary Fig. S3b) , . and based on a Nanowizard 4 (JPK Instruments, Bruker) mounted on a Zeiss inverted optical microscope.

Techniques: Fluorescence, Microscopy, Construct

Journal: bioRxiv

Article Title: Phase separation and molecular ordering of the prion-like domain of the thermosensory protein EARLY FLOWERING 3

doi: 10.1101/2023.03.12.532276

Figure Lengend Snippet: Wide-field total internal reflection fluorescence microscopy and high-resolution AFM of ELF3-GFP PrLD gel droplets. A) Young’s modulus measurements of the droplets showing in-homogeneous stiffness within individual droplets, scale bar = 1 μm. B) Fluorescence images of ELF3 PrLD Q0 (left), Q7 (middle) and Q20 (right), scalebar = 1 μm. Areas of interest are boxed and shown in close-up in (c). C) AFM topography of the droplets in a, scalebar = 1 μm. D) Height profile measurements across individual droplets (blue lines in c) showing a step pattern between flat layers.

Article Snippet: AFM coupled wide-field fluorescence microscope was developed in-house, allowing AFM imaging and epifluorescence or Total Internal Reflection Fluorescence (TIRF) imaging (Supplementary Fig. S3b) , . and based on a Nanowizard 4 (JPK Instruments, Bruker) mounted on a Zeiss inverted optical microscope.

Techniques: Fluorescence, Microscopy